Your Friendly Neighborhood Spider-Man depicts Spidey’s formative years in a separate universe as the in MCU movies. With this backdrop, Spider-Man will face a range of villains, from classic threats to new, unexpected enemies, offering fresh storytelling opportunities.

21 Lonnie Lincoln, AKA Tombstone

First Appeared In Episode 1 “Amazing Fantasy”

Lonnie Lincoln is initially portrayed as a high school athlete and one of Peter’s classmates, where the pair quickly become firm friends. However, after a string of rotten luck, Lonnie becomes embroiled in the 110th Street Gang. Though it hasn’t yet occurred in Your Friendly Neighborhood Spider-Man, in the comics, Lonnie Lincoln eventually becomes the fearsome crime lord known as Tombstone. Unlike other villains who rely on technology or science, Tombstone represents the gritty, street-level threats Spider-Man must face. His story arc will likely show his continued descent into crime.

20 The Symbiotes

First Appeared In Episode 1 “Amazing Fantasy”

While Venom is not yet confirmed, the symbiotes were established early in Your Friendly Neighborhood Spider-Man during the opening scenes. Doctor Strange is depicted battling a symbiote monster when Peter Parker heads to his new school. The alien parasites bond with hosts, amplifying their abilities while corrupting their minds. This could pave the way for Spider-Man’s famous black suit saga or introduce the symbiotes Venom and Carnage. Their inclusion expands the series beyond Earth-based threats, bringing in cosmic horror elements.

19 Norman Osborn, AKA Green Goblin

First Appeared In Episode 1 “Amazing Fantasy”

Norman Osborn is one of the most complex and dangerous figures in Spider-Man’s history. In Your Friendly Neighborhood Spider-Man, he serves as Peter’s mentor, but his darker side lurks beneath the surface. As the head of Oscorp, he conducts dangerous experiments that will presumably ultimately lead to his transformation into the Green Goblin. His dual role as both a father figure and a villain adds emotional weight to his conflict with Spider-Man, making their inevitable clash even more tragic and impactful within the MCU’s new Spider-Man universe.

18 Harry Osborn, AKA Green Goblin

First Appeared In Episode 1 “Amazing Fantasy”

Harry Osborn, Peter Parker’s best friend and Norman’s son, has always struggled with his father’s legacy. In Your Friendly Neighborhood Spider-Man, Harry is a vain influencer who is rescued in episode 1 by Spider-Man. He then becomes part of the Spider-Man team, working alongside his father. In the comics, Harry eventually takes over his father’s mantle as the second Green Goblin. This sets up a compelling personal conflict between Peter and Harry, forcing Spider-Man to fight not just a villain but a friend torn between loyalty and a path of destruction.

17 Carla Connors, AKA The Lizard

First Appeared In Episode 2 “The Parker Luck"

A new spin on the Lizard, Carla Conners, is a gender-swapped version of Curt Connors. As an Oscorp scientist, she oversees Peter Parker’s work as one of Osborne’s interns. In the comics, Connors experiments with reptilian DNA, hoping to find breakthroughs in human regeneration. However, after testing the formula, he transforms into a monstrous, uncontrollable Lizard. Her role in Your Friendly Neighborhood Spider-Man is fairly minor thus far, but the arrival of reptiles in episode 8 foreshadows her transformation.

16 Bentley Wittman, AKA Wizard

First Appeared In Episode 2 “The Parker Luck"

Bentley Wittman is a brilliant but egotistical scientist working at Oscorp, known for his arrogance and disregard for ethics. In the comics, his technological genius allows him to create advanced weaponry and gadgets, eventually leading him to take on the identity of the Wizard. In Your Friendly Neighborhood Spider-Man, Wittman appears as a grumpy, rude, and condescending scientist who oversees the Oscorp interns. Though a far cry from his villainous comic book counterpart, Wittman remains one of the most dislikeable characters.

15 Butane, The Arsonist

First Appeared In Episode 2 “The Parker Luck"

In Marvel Comics, Butane is transformed into a fiery humanoid and is trained as part of a superhuman army. In Your Friendly Neighborhood Spider-Man, Butane is pesented as an arsonist, who has been hired to burn down a building as part of an insurance scam, using his high-tech flamethrowers. After he accidentally destroys the wrong building, Spider-Man defeats Butane, whereupon Spider-Man notices a logo on the weapons, which he’ll later realize is Otto Octavius’s.

14 James Sanders, AKA Speed Demon

First Appeared In Episode 3 “Secret Identity Crisis"

James Sanders is a petty criminal, who in the comics is known as Speed Demon. He typically possesses the power of superhuman speed, enabling him to move and react with lightning-fast reflexes. His speed makes him a formidable foe in battle, capable of outpacing Spider-Man and dodging his attacks with ease. In Your Friendly Neighborhood Spider-Man, James receives high-tech boots from Octavius that give him his powers, working alongside his partner Maria to enact a jewelry store robbery and battle Web-Head.

13 Maria Vasquez, AKA Tarantula

First Appeared In Episode 3 “Secret Identity Crisis"

Maia Vasquez is the criminal working with James Sanders who receives technology from Octavius. Maria dons gloves that bestow her with energy projection abilities, which she uses with devastating effect while fighting Spider-Man in Your Friendly Neighborhood Spider-Man. In Marvel Comics, Maria Vasquez is one of several characters known as Tarantula. While Maria Vasquez is more of a heroic figure, working for the Heroes For Hire, most Tarantulas have been villains.

12 Benjamin "Big" Donovan

First Appeared In Episode 3 “Secret Identity Crisis"

In Marvel Comics, Big Donovan is a classic enforcer-type villain, often found in the criminal underworld. He's a physically imposing figure, using his size and strength to intimidate and overpower his enemies. In Your Friendly Neighborhood Spider-Man, Big Donovan is the hulking leader of the 110th Street Gang. He has a formidable stature that towers over his enemies. In the comics, Big Donovan works for Tombstone, suggesting there may be a shift in the power dynamic to come.